These authors contributed equally to this work.
Supramolecular Host–Guest Interaction for Labeling and Detection of Cellular Biomarkers†
Article first published online: 24 NOV 2011
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 2, pages 450–454, January 9, 2012
How to Cite
Agasti, S. S., Liong, M., Tassa, C., Chung, H. J., Shaw, S. Y., Lee, H. and Weissleder, R. (2012), Supramolecular Host–Guest Interaction for Labeling and Detection of Cellular Biomarkers . Angew. Chem. Int. Ed., 51: 450–454. doi: 10.1002/anie.201105670
We thank M. Fernandez-Suarez, R. Mazitschek, T. Reiner, K. S. Yang, and K. Tran for helpful discussions; I. Bagayev and E. Tiglao for assistance in cell experiments; N. Sergeyev for providing the cross-linked iron oxide particles; Y. Fisher-Jeffes for reviewing the manuscript. This work was supported in part by National Institutes of Health Grants R01-EB0044626, R01-EB010011, T32 grant T32A79443, P50 grant P50A86355, CCNE contract U54A151884, and TPEN contract HHSN268201000044C.
- Issue published online: 4 JAN 2012
- Article first published online: 24 NOV 2011
- Manuscript Revised: 7 OCT 2011
- Manuscript Received: 10 AUG 2011
- National Institutes of Health. Grant Numbers: R01-EB0044626, R01-EB010011, T32A79443, P50A86355, U54A151884, HHSN268201000044C
- bioorthogonal labeling;
- host–guest systems;
- supramolecular chemistry
Be my guest: A supramolecular host–guest interaction is utilized for highly efficient bioorthogonal labeling of cellular targets. Antibodies labeled with a cyclodextrin host molecule bind to adamantane-labeled magnetofluorescent nanoparticles (see picture) and provide an amplifiable strategy for biomarker detection that can be adapted to different diagnostic techniques such as molecular profiling or magnetic cell sorting.