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Supramolecular Host–Guest Interaction for Labeling and Detection of Cellular Biomarkers

Authors

  • Dr. Sarit S. Agasti,

    1. Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA)
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    • These authors contributed equally to this work.

  • Dr. Monty Liong,

    1. Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA)
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    • These authors contributed equally to this work.

  • Dr. Carlos Tassa,

    1. Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA)
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  • Dr. Hyun Jung Chung,

    1. Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA)
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  • Prof. Stanley Y. Shaw,

    1. Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA)
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  • Prof. Hakho Lee,

    Corresponding author
    1. Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA)
    • Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA)
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  • Prof. Ralph Weissleder

    Corresponding author
    1. Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA)
    2. Department of Systems Biology, Harvard Medical School, 200 Longwood Ave., Alpert 536, Boston, MA 02115 (USA)
    • Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, 185 Cambridge St., Boston, MA 02114 (USA)
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  • We thank M. Fernandez-Suarez, R. Mazitschek, T. Reiner, K. S. Yang, and K. Tran for helpful discussions; I. Bagayev and E. Tiglao for assistance in cell experiments; N. Sergeyev for providing the cross-linked iron oxide particles; Y. Fisher-Jeffes for reviewing the manuscript. This work was supported in part by National Institutes of Health Grants R01-EB0044626, R01-EB010011, T32 grant T32A79443, P50 grant P50A86355, CCNE contract U54A151884, and TPEN contract HHSN268201000044C.

Abstract

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Be my guest: A supramolecular host–guest interaction is utilized for highly efficient bioorthogonal labeling of cellular targets. Antibodies labeled with a cyclodextrin host molecule bind to adamantane-labeled magnetofluorescent nanoparticles (see picture) and provide an amplifiable strategy for biomarker detection that can be adapted to different diagnostic techniques such as molecular profiling or magnetic cell sorting.

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