These authors contributed equally to this work.
Article first published online: 14 NOV 2011
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 1, pages 254–257, January 2, 2012
How to Cite
Baccaro, A., Steck, A.-L. and Marx, A. (2012), Barcoded Nucleotides. Angew. Chem. Int. Ed., 51: 254–257. doi: 10.1002/anie.201105717
We gratefully acknowledge support by the Konstanz Research School Chemical Biology; the group of C. Hauck, University of Konstanz, for providing equipment, and the Ministerium für Wissenschaft, Forschung und Kunst, Baden-Württemberg for funding within the programme Bionik.
- Issue published online: 29 DEC 2011
- Article first published online: 14 NOV 2011
- Manuscript Revised: 15 SEP 2011
- Manuscript Received: 12 AUG 2011
- Ministerium für Wissenschaft, Forschung und Kunst, Baden-Württemberg
- DNA polymerase;
- enzymatic synthesis;
Designer labels: DNA polymerases are able to incorporate oligodeoxynucleotide(ODN)-modified nucleotides that are 40-times bigger than the natural substrate. The result is bio-barcoded DNA (see scheme). Incorporation of an ODN-modified nucleotide can be detected in solution and on solid support by hybridization of a fluorescent-dye-labeled DNA strand complementary to the ODN.