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Keywords:

  • DNA polymerase;
  • enzymatic synthesis;
  • microarray;
  • nucleotides;
  • oligonucleotides
Thumbnail image of graphical abstract

Designer labels: DNA polymerases are able to incorporate oligodeoxynucleotide(ODN)-modified nucleotides that are 40-times bigger than the natural substrate. The result is bio-barcoded DNA (see scheme). Incorporation of an ODN-modified nucleotide can be detected in solution and on solid support by hybridization of a fluorescent-dye-labeled DNA strand complementary to the ODN.