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Molecular Details of the Recognition of Blood Group Antigens by a Human Norovirus as Determined by STD NMR Spectroscopy

Authors

  • Brigitte Fiege,

    1. Center of Structural and Cell Biology in Medicine, Institute of Chemistry, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck (Germany)
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  • Dr. Christoph Rademacher,

    1. Center of Structural and Cell Biology in Medicine, Institute of Chemistry, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck (Germany)
    2. Present address: Department of Chemical Physiology and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037 (USA)
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  • Jonathan Cartmell,

    1. Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2 (Canada)
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  • Dr. Pavel I. Kitov,

    1. Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2 (Canada)
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  • Prof. Dr. Francisco Parra,

    1. Instituto Universitario de Biotecnología de Asturias, Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33006 Oviedo (Spain)
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  • Prof. Dr. Thomas Peters

    Corresponding author
    1. Center of Structural and Cell Biology in Medicine, Institute of Chemistry, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck (Germany)
    • Center of Structural and Cell Biology in Medicine, Institute of Chemistry, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck (Germany)
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  • T.P. acknowledges the DFG for grant Pe494/8-1 and for grants HBFG 101/192-1 and ME 1830/1. B.F. thanks the Studienstiftung des deutschen Volkes for a stipend and C.R. thanks the Verband der Chemischen Industrie (VCI) for a stipend. F.P. thanks the Spanish Ministerio de Ciencia e Innovación for grant BIO2009-07535 and the European Union FEDER program for support. The work of P.I.K. was supported by the Alberta Ingenuity Centre for Carbohydrate Science and the Natural Science and Engineering Research Council of Canada. STD= saturation transfer difference.

Abstract

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Tracing the infection: The binding of human norovirus particles to blood group antigens was investigated using NMR spectroscopy. Binding epitopes were determined at atomic resolution, information on the binding specificity was obtained, and the bioactive conformation of various sugars was revealed. This provides valuable information for the design of entry inhibitors against this important class of human pathogenic viruses.

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