This work was supported by PICS 4862 (CNRS, CNRST), Institut Pasteur de Lille, Université Lille Nord de France, Cancéropôle Nord Ouest, Région Nord pas de Calais, and European Community.
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Communication
A One-Pot Three-Segment Ligation Strategy for Protein Chemical Synthesis†
Article first published online: 16 NOV 2011
DOI: 10.1002/anie.201105837
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Ollivier, N., Vicogne, J., Vallin, A., Drobecq, H., Desmet, R., El Mahdi, O., Leclercq, B., Goormachtigh, G., Fafeur, V. and Melnyk, O. (2012), A One-Pot Three-Segment Ligation Strategy for Protein Chemical Synthesis. Angew. Chem. Int. Ed., 51: 209–213. doi: 10.1002/anie.201105837
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Publication History
- Issue published online: 29 DEC 2011
- Article first published online: 16 NOV 2011
- Manuscript Received: 18 AUG 2011
Keywords:
- growth factors;
- native chemical ligation;
- proteins;
- solid-phase synthesis;
- synthetic methods
Three in one: Native chemical ligation (NCL) and bis(2-sulfanylethyl)amido (SEA) ligation allow the one-pot assembly of three peptide segments in the N-to-C direction. The SEA group (see picture, blue) is switched off by intramolecular disulfide bond formation during NCL. Then, a phosphine switches it on to trigger the second SEA ligation step. The K1 domain of the hepatocyte growth factor was synthesized and found to be biologically active.