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Communication

Solid-State NMR Spectroscopy on Cellular Preparations Enhanced by Dynamic Nuclear Polarization†

  1. Dr. Marie Renault1,
  2. Dr. Shane Pawsey3,
  3. Dr. Martine P. Bos2,
  4. Eline J. Koers1,
  5. Dr. Deepak Nand1,
  6. Ria Tommassen-van Boxtel2,
  7. Dr. Melanie Rosay3,
  8. Prof. Jan Tommassen2,
  9. Dr. Werner E. Maas3,
  10. Prof. Marc Baldus1,*

Article first published online: 1 FEB 2012

DOI: 10.1002/anie.201105984

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 51, Issue 12, pages 2998–3001, March 19, 2012

Additional Information

How to Cite

Renault, M., Pawsey, S., Bos, M. P., Koers, E. J., Nand, D., Tommassen-van Boxtel, R., Rosay, M., Tommassen, J., Maas, W. E. and Baldus, M. (2012), Solid-State NMR Spectroscopy on Cellular Preparations Enhanced by Dynamic Nuclear Polarization . Angew. Chem. Int. Ed., 51: 2998–3001. doi: 10.1002/anie.201105984

Author Information

  1. 1

    Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht (The Netherlands)

  2. 2

    Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht (The Netherlands)

  3. 3

    Bruker BioSpin Corp., Billerica, MA 01821 (USA)

*Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht (The Netherlands)

  1. †

    This study was presented in part at the 52nd ENC conference, Asilomar, CA, April 10–15, 2011. Funding by NWO (grants 700.26.121 and 815.02.012) and the European Community’s Seventh Framework Program [FP7/2007-2013] under Grant Agreement no. 211800 is gratefully acknowledged.

Publication History

  1. Issue published online: 13 MAR 2012
  2. Article first published online: 1 FEB 2012
  3. Manuscript Revised: 2 NOV 2011
  4. Manuscript Received: 24 AUG 2011

Funded by

  • NWO. Grant Numbers: 700.26.121, 815.02.012
  • European Community’s Seventh Framework Program. Grant Numbers: FP7/2007-2013, 211800

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Keywords:

  • analytical methods;
  • dynamic nuclear polarization;
  • membrane proteins;
  • RNA;
  • solid-state NMR spectroscopy
Thumbnail image of graphical abstract

A peek inside: Dynamic nuclear polarization (DNP) enhances the spectroscopic sensitivity of solid-state NMR measurements of uniformly (13C,15N)-labeled preparations of Escherichia coli cells by more than an order of magnitude (see picture; MW=microwaves, ε=enhancement factor). The major molecular components in the cells can be characterized in this way.

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