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Solid-State NMR Spectroscopy on Cellular Preparations Enhanced by Dynamic Nuclear Polarization


  • This study was presented in part at the 52nd ENC conference, Asilomar, CA, April 10–15, 2011. Funding by NWO (grants 700.26.121 and 815.02.012) and the European Community’s Seventh Framework Program [FP7/2007-2013] under Grant Agreement no. 211800 is gratefully acknowledged.


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A peek inside: Dynamic nuclear polarization (DNP) enhances the spectroscopic sensitivity of solid-state NMR measurements of uniformly (13C,15N)-labeled preparations of Escherichia coli cells by more than an order of magnitude (see picture; MW=microwaves, ε=enhancement factor). The major molecular components in the cells can be characterized in this way.