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Keywords:

  • erythropoietin;
  • kinetically controlled ligation;
  • native chemical ligation;
  • proteins;
  • solid-phase synthesis
Thumbnail image of graphical abstract

A new route to EPO: Nonglycosylated human erythropoietin (EPO) was prepared using a convergent chemical ligation synthetic strategy. The synthetic [Lysine24, 38, 83] EPO analogue, which was purified by HPLC and has the correct molecular weight (see picture), shows well-defined covalent structure, is correctly folded, and is biologically active in a factor-dependent cell line assay.