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Creation of a Lipase Highly Selective for trans Fatty Acids by Protein Engineering

Authors

  • Dr. Henrike B. Brundiek,

    1. Institut für Biochemie, Abt. Biotechnologie & Enzymkatalyse, Universität Greifswald, Felix-Hausdorff-Strasse 4, 17487 Greifswald (Germany)
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  • Dr. Andrew S. Evitt,

    1. Institut für Biochemie, Abt. Biotechnologie & Enzymkatalyse, Universität Greifswald, Felix-Hausdorff-Strasse 4, 17487 Greifswald (Germany)
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  • Dr. Robert Kourist,

    1. Institut für Biochemie, Abt. Biotechnologie & Enzymkatalyse, Universität Greifswald, Felix-Hausdorff-Strasse 4, 17487 Greifswald (Germany)
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  • Prof. Dr. Uwe T. Bornscheuer

    Corresponding author
    1. Institut für Biochemie, Abt. Biotechnologie & Enzymkatalyse, Universität Greifswald, Felix-Hausdorff-Strasse 4, 17487 Greifswald (Germany)
    • Institut für Biochemie, Abt. Biotechnologie & Enzymkatalyse, Universität Greifswald, Felix-Hausdorff-Strasse 4, 17487 Greifswald (Germany)
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Abstract

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Sorting out: Protein engineering of lipase CAL-A led to the discovery of mutants with excellent chemoselectivity for the removal of trans and saturated fatty acids from partially hydrogenated vegetable oil. These fatty acids, identified as a major risk factor for human health, can now be removed by enzyme catalysis.

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