We thank Dr. A. Kloxin and M. Tibbitt for useful discussions on photopatterning. Fellowship assistance to C.A.D. was awarded by the US Department of Education Graduate Assistantships in Areas of National Need. This work was supported financially by the National Science Foundation (DMR 1006711) and the Howard Hughes Medical Institute.
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Photoreversible Patterning of Biomolecules within Click-Based Hydrogels†
Article first published online: 8 DEC 2011
DOI: 10.1002/anie.201106463
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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How to Cite
DeForest, C. A. and Anseth, K. S. (2012), Photoreversible Patterning of Biomolecules within Click-Based Hydrogels. Angew. Chem. Int. Ed., 51: 1816–1819. doi: 10.1002/anie.201106463
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Publication History
- Issue published online: 14 FEB 2012
- Article first published online: 8 DEC 2011
- Manuscript Revised: 16 NOV 2011
- Manuscript Received: 13 SEP 2011
Funded by
- Funded Access
- US Department of Education
- National Science Foundation. Grant Number: DMR 1006711
- Howard Hughes Medical Institute
Keywords:
- bioorganic chemistry;
- hydrogels;
- peptides;
- photochemistry;
- RGD peptides
Lighting the way: Biochemical cues have been reversibly patterned into hydrogels with full spatiotemporal control by using two photochemical reactions. The hydrogel is conjugated with a peptide by a thiol-ene photoaddition reaction that is initiated by visible light. Subsequent, selective photocleavage of an o-nitrobenzyl ether with UV light enables dynamic presentation of the peptide to cells with control in three dimensions.