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Keywords:

  • cinchona alkaloids;
  • DNA structures;
  • liquid chromatography;
  • molecular recognition;
  • plasmid topoisomers
Thumbnail image of graphical abstract

Recognizing pDNA supercoils: Differently supercoiled species of the same plasmid DNA can be separated by topology-selective chromatography. Two-dimensional HPLC proved that the supercoiling changes during fermentation. Thus, a new quality criterium might help to optimize the effectivity of future genetic drugs and vaccines.