Topology-Selective Chromatography Reveals Plasmid Supercoiling Shifts during Fermentation and Allows Rapid and Efficient Preparation of Topoisomers

Authors


  • We thank Boehringer-Ingelheim RCV (Vienna, Austria) for financial support and for providing plasmid material and fermentation samples. We also thank Andreas Reininger for help with AFM images.

Abstract

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Recognizing pDNA supercoils: Differently supercoiled species of the same plasmid DNA can be separated by topology-selective chromatography. Two-dimensional HPLC proved that the supercoiling changes during fermentation. Thus, a new quality criterium might help to optimize the effectivity of future genetic drugs and vaccines.

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