Site-Specific Conjugation of ScFvs Antibodies to Nanoparticles by Bioorthogonal Strain-Promoted Alkyne–Nitrone Cycloaddition


  • M.C. and S.M. acknowledge the research fellowships of CMENA. This work was supported by NanoMeDia Project (Regione Lombardia) and “Fondazione Romeo e Enrica Invernizzi”.


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Particularly suitable: An N-terminal serine mutant of anti-HER2 scFv antibody was conjugated to polymer-coated magnetofluorescent nanoparticles by strain-promoted alkyne–nitrone cycloaddition. The resulting nanoparticles (see scheme) proved effective in targeting and labeling HER2-positive breast cancer cells.