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Phosphopeptide-Dependent Labeling of 1433 ζ Proteins by Fusicoccin-Based Fluorescent Probes

Authors

  • Michiko Takahashi,

    1. The Institute of Scientific and Industrial Research (ISIR), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
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  • Akie Kawamura,

    1. The Institute of Scientific and Industrial Research (ISIR), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
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  • Prof. Nobuo Kato,

    1. The Institute of Scientific and Industrial Research (ISIR), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
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  • Prof. Tsuyoshi Nishi,

    1. The Institute of Scientific and Industrial Research (ISIR), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
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  • Prof. Itaru Hamachi,

    1. Department of Synthetic Chemistry and Biological Chemistry Graduate School of Engineering, Kyoto University (Japan)
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  • Prof. Junko Ohkanda

    Corresponding author
    1. The Institute of Scientific and Industrial Research (ISIR), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
    • The Institute of Scientific and Industrial Research (ISIR), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan)
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  • This work was supported by the Japan Society for the Promotion of Science (KAKENHI no22655055, J.O.) and the Eisai Research Foundation (J.O.), and was partially supported by the Program for the Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation of Japan (N.K.). We thank Prof. S. Uchiyama and Dr. M. Noda (Osaka University) for their assistance on MALDI-TOF mass measurements.

Abstract

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Fluorescent combination: Cell-penetrating probes derived from the diterpene fusicoccin can form ternary complexes with 14–3–3 proteins and phosphopeptide ligands, whereupon the probes site-specifically attach a fluorescent tag onto the surface of the 14–3–3 proteins.

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