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Genetically Encoded Photocrosslinkers as Molecular Probes To Study G-Protein-Coupled Receptors (GPCRs)


  • We are grateful to Dr. Michael Hoesl for the critical reading of this manuscript.


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The genetic code was expanded with orthogonal pairs to introduce photoactivatable amino acids into G-protein-coupled receptors (GPCRs) in a noninvasive manner. In this way the receptor surface could be mapped by searching for specific ligand interaction sites and the complex dynamics could be studied. This method is also useful for probing the structure of GPCR complexes in living cells.