An Activity-Based Imaging Probe for the Integral Membrane Hydrolase KIAA1363

Authors

  • Jae Won Chang,

    1. Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037(USA)
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    • These authors contributed equally to this work.

  • Dr. Raymond E. Moellering,

    1. Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037(USA)
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    • These authors contributed equally to this work.

  • Prof. Dr. Benjamin F. Cravatt

    Corresponding author
    1. Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037(USA)
    • Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037(USA)
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  • We would like to thank A. Adibekian and the Cravatt lab for insightful discussions and W. Kiosses and the TSRI Microscopy Core for technical assistance. This research was supported by the California Institute for Regenerative Medicine (predoctoral fellowship to J.W.C.), the American Association for Cancer Research (Centennial predoctoral fellowship to R.E.M.), the Damon Runyon Cancer Research Foundation (HHMI postdoctoral fellowship to R.E.M.), the US National Institutes of Health (grant number CA087660), and the Skaggs Institute for Chemical Biology.

Abstract

original image

Profiling enzyme activities: A selective, activity-based imaging probe is described for the integral membrane hydrolase KIAA1363 and used to determine the subcellular localization and half-life of this enzyme in human cancer cells (see picture).

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