Ambitious undertaking: The β-subunit of the human follicle-stimulating hormone (hFSH) displaying a N-linked consensus sequence oligosaccharide at each of the two wild-type sites was synthesized. The glycoprotein has been designed with acetamidomethyl protected cysteine residues, anticipating folding and association with the α-subunit. This represents the largest realistically glycosylated homogeneous glycoprotein obtained by using strictly chemical methods.
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