Communication

An O-Centered Structure of the Trinuclear Copper Center in the Cys500Ser/Glu506Gln Mutant of CueO and Structural Changes in Low to High X-Ray Dose Conditions†

  1. Prof. Hirofumi Komori2,3,
  2. Ryosuke Sugiyama1,
  3. Prof. Kunishige Kataoka1,
  4. Prof. Yoshiki Higuchi2,3,
  5. Prof. Takeshi Sakurai1,*

Article first published online: 16 JAN 2012

DOI: 10.1002/anie.201107739

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 51, Issue 8, pages 1861–1864, February 20, 2012

Additional Information

How to Cite

Komori, H., Sugiyama, R., Kataoka, K., Higuchi, Y. and Sakurai, T. (2012), An O-Centered Structure of the Trinuclear Copper Center in the Cys500Ser/Glu506Gln Mutant of CueO and Structural Changes in Low to High X-Ray Dose Conditions. Angew. Chem. Int. Ed., 51: 1861–1864. doi: 10.1002/anie.201107739

Author Information

  1. 1

    Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa 920-1192 (Japan)

  2. 2

    Graduate School of Life Science, University of Hyogo, 3-2-1, Koto, Kamigori-cho, Ako-gun 678-1297 (Japan)

  3. 3

    RIKEN Spring-8 Center, 1-1-1 Koto, Mikazuki-cho, Sayo-gun, 679-5248 (Japan)

*Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa 920-1192 (Japan)

  1. †

    We acknowledge financial support from a Grand-in-Aid for Scientific Research (23580131) from the Ministry of Education, Science, Sports and Culture of Japan (to K.K.). We also thank the beamline staff at BL26B2 for their kind help with X-ray data collection.

Publication History

  1. Issue published online: 14 FEB 2012
  2. Article first published online: 16 JAN 2012
  3. Manuscript Received: 3 NOV 2011

Funded by

  • Ministry of Education, Science, Sports and Culture of Japan. Grant Number: 23580131

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article with Supporting Information (HTML) Get PDF (593K)

Keywords:

  • bioinorganic chemistry;
  • enzyme catalysis;
  • metalloproteins;
  • X-ray absorption spectroscopy;
  • X-ray diffraction
Thumbnail image of graphical abstract

Right on CueO: The O-centered structure of the trinuclear copper center in a multicopper oxidase (CueO) was shown to be an intermediate of the four-electron reduction of dioxygen (see picture). This structure was determined by in situ data collection of X-ray diffractions and copper K-edge spectra at low to high X-ray dose conditions.

View Full Article with Supporting Information (HTML) Get PDF (593K)