This work was supported by the Intramural Research Program of the NIBIB, NIH and partially supported by an NIH Pathway to Independence (K99/R00) Award, the Henry M. Jackson Foundation, and the Intramural Research Program of KIST (Korea). We thank Myung Sun Lee for illustrations.
Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad-Spectrum Nanoquencher†
Article first published online: 2 JAN 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 7, pages 1625–1630, February 13, 2012
How to Cite
Huang, X., Swierczewska, M., Choi, K. Y., Zhu, L., Bhirde, A., Park, J., Kim, K., Xie, J., Niu, G., Lee, K. C., Lee, S. and Chen, X. (2012), Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad-Spectrum Nanoquencher. Angew. Chem. Int. Ed., 51: 1625–1630. doi: 10.1002/anie.201107795
- Issue published online: 7 FEB 2012
- Article first published online: 2 JAN 2012
- Manuscript Revised: 13 DEC 2011
- Manuscript Received: 5 NOV 2011
- NIBIB, NIH
- Henry M. Jackson Foundation
- imaging agents;
Turn off the lights! A universal nanoquencher that quenches a broad range of visible to near-infrared dyes by using a series of dark quenchers incorporated into a cell-permeable mesoporous silica nanoparticle has been developed. In combination with dye-labeled substrates, this nanoquencher boosts multiple fluorescence signals upon specific proteolysis, which allows real-time imaging of proteolytic cascades (see scheme).