Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad-Spectrum Nanoquencher

Authors

  • Dr. Xinglu Huang,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
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  • Magdalena Swierczewska,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
    2. Department of Biomedical Engineering, Stony Brook University (USA)
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  • Dr. Ki Young Choi,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
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  • Dr. Lei Zhu,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
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  • Dr. Ashwinkumar Bhirde,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
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  • Dr. Jinwoo Park,

    1. BioActs, DKC Corporation (Korea)
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  • Dr. Kwangmeyung Kim,

    1. Center for Theragnosis, Korea Institute of Science and Technology (KIST, Korea)
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  • Prof. Jin Xie,

    1. Department of Chemistry and Bio-Imaging Research Center, University of Georgia (USA)
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  • Dr. Gang Niu,

    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
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  • Prof. Kang Choon Lee,

    1. College of Pharmacy, SungKyunKwan University (Korea)
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  • Dr. Seulki Lee,

    Corresponding author
    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
    • Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
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  • Dr. Xiaoyuan Chen

    Corresponding author
    1. Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
    • Laboratory of Molecular Imaging and Nanomedicine (LOMIN) National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH, USA)
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  • This work was supported by the Intramural Research Program of the NIBIB, NIH and partially supported by an NIH Pathway to Independence (K99/R00) Award, the Henry M. Jackson Foundation, and the Intramural Research Program of KIST (Korea). We thank Myung Sun Lee for illustrations.

Abstract

original image

Turn off the lights! A universal nanoquencher that quenches a broad range of visible to near-infrared dyes by using a series of dark quenchers incorporated into a cell-permeable mesoporous silica nanoparticle has been developed. In combination with dye-labeled substrates, this nanoquencher boosts multiple fluorescence signals upon specific proteolysis, which allows real-time imaging of proteolytic cascades (see scheme).

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