Communication

Ratiometric Fluorescence Detection of Pathogenic Bacteria Resistant to Broad-Spectrum β-Lactam Antibiotics

  1. Dr. Junxiang Zhang1,
  2. Yang Shen1,
  3. Sarah L. May1,
  4. Prof. Daniel C. Nelson1,3,
  5. Prof. Shuwei Li1,2,*

Article first published online: 13 JAN 2012

DOI: 10.1002/anie.201107810

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 51, Issue 8, pages 1865–1868, February 20, 2012

Additional Information

How to Cite

Zhang, J., Shen, Y., May, S. L., Nelson, D. C. and Li, S. (2012), Ratiometric Fluorescence Detection of Pathogenic Bacteria Resistant to Broad-Spectrum β-Lactam Antibiotics. Angew. Chem. Int. Ed., 51: 1865–1868. doi: 10.1002/anie.201107810

Author Information

  1. 1

    Institute for Bioscience and Biotechnology Research, University of Maryland College Park, 9600 Gudelsky Drive, Rockville, MD 20850 (USA)

  2. 2

    Department of Chemistry and Biochemistry, University of Maryland College Park (USA)

  3. 3

    Department of Veterinary Medicine, University of Maryland College Park (USA)

*Institute for Bioscience and Biotechnology Research, University of Maryland College Park, 9600 Gudelsky Drive, Rockville, MD 20850 (USA)

Publication History

  1. Issue published online: 14 FEB 2012
  2. Article first published online: 13 JAN 2012
  3. Manuscript Received: 6 NOV 2011

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Keywords:

  • antibiotics;
  • cephalosporins;
  • drug-resistant bacteria;
  • fluorescence;
  • β-lactamases
Thumbnail image of graphical abstract

Identification of bacteria: A methoxyimino cephalosporin derivative containing a pair of fluorescence resonance energy transfer (FRET) fluorophores was synthesized. This probe displays selective cleavage toward different types of β-lactamases, thereby providing a rapid assay to distinguish bacterial cells that are either sensitive or resistant to broad-spectrum β-lactam antibiotics (see picture).

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