Get access

In Vitro and In Vivo Uncaging and Bioluminescence Imaging by Using Photocaged Upconversion Nanoparticles

Authors

  • Yanmei Yang,

    1. Division of Chemistry and Biological Chemistry, School of Physical & Mathematical Sciences, Nanyang Technological University, Singapore, 637371 (Singapore)
    Search for more papers by this author
  • Qing Shao,

    1. Division of Chemistry and Biological Chemistry, School of Physical & Mathematical Sciences, Nanyang Technological University, Singapore, 637371 (Singapore)
    Search for more papers by this author
  • Renren Deng,

    1. Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543 (Singapore)
    Search for more papers by this author
  • Chao Wang,

    1. Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Institute of Functional Nano & Soft Materials, Soochow University (P. R. China)
    Search for more papers by this author
  • Xue Teng,

    1. Division of Biomedical Engineering, School of Chemical and Biomedical Engineering, Nanyang Technological University (Singapore)
    Search for more papers by this author
  • Kai Cheng,

    1. Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University Medical Center (USA)
    Search for more papers by this author
  • Prof. Zhen Cheng,

    1. Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University Medical Center (USA)
    Search for more papers by this author
  • Prof. Ling Huang,

    1. Division of Biomedical Engineering, School of Chemical and Biomedical Engineering, Nanyang Technological University (Singapore)
    Search for more papers by this author
  • Prof. Zhuang Liu,

    1. Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Institute of Functional Nano & Soft Materials, Soochow University (P. R. China)
    Search for more papers by this author
  • Prof. Xiaogang Liu,

    Corresponding author
    1. Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543 (Singapore)
    2. Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 3 Research Link, Singapore 117602 (Singapore)
    • Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543 (Singapore)
    Search for more papers by this author
  • Prof. Bengang Xing

    Corresponding author
    1. Division of Chemistry and Biological Chemistry, School of Physical & Mathematical Sciences, Nanyang Technological University, Singapore, 637371 (Singapore)
    • Division of Chemistry and Biological Chemistry, School of Physical & Mathematical Sciences, Nanyang Technological University, Singapore, 637371 (Singapore)
    Search for more papers by this author

  • This work was supported in part by NTU Start-Up Grant (SUG), Tier 1 (RG 64/10), the Singapore-Perking-Oxford Research Enterprise (SPORE), and the Singapore Ministry of Education (MOE2010-T2-083).

Abstract

original image

Trading up: A bioimaging system that is based on caged D-luciferin/upconversion nanoparticle conjugate has been developed. The nanoparticles upconvert near-infrared light into UV light, which triggers the photorelease of D-luciferin (see scheme) and leads to enhanced fluorescence and bioluminescence signals in vitro and in vivo. The use of near-infrared light enables deep penetration into tissue in vivo with minimum cellular damage.

Get access to the full text of this article

Ancillary