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Keywords:

  • analytical methods;
  • fluorescence;
  • FRET;
  • imaging agents;
  • sensors
Thumbnail image of graphical abstract

Traffic cam: A tandem dye prepared from a FRET acceptor and a fluorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization serves to follow protein trafficking during endocytosis. This sensor was used to analyze agonist-dependent internalization of β2-adrenergic receptors (see scheme). It was also used as a surrogate antigen to reveal direct surface-to-endosome antigen transfer between dendritic cells (not shown).