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Communication

Genetically Encoded pH Sensor for Tracking Surface Proteins through Endocytosis†

  1. Anmol Grover2,
  2. Dr. Brigitte F. Schmidt3,
  3. Dr. Russell D. Salter4,
  4. Dr. Simon C. Watkins4,
  5. Dr. Alan S. Waggoner2,
  6. Dr. Marcel P. Bruchez1,*

Article first published online: 29 MAR 2012

DOI: 10.1002/anie.201108107

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 51, Issue 20, pages 4838–4842, May 14, 2012

Additional Information

How to Cite

Grover, A., Schmidt, B. F., Salter, R. D., Watkins, S. C., Waggoner, A. S. and Bruchez, M. P. (2012), Genetically Encoded pH Sensor for Tracking Surface Proteins through Endocytosis. Angew. Chem. Int. Ed., 51: 4838–4842. doi: 10.1002/anie.201108107

Author Information

  1. 1

    Department of Chemistry, Department of Biological Sciences and Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213 (USA)

  2. 2

    Department of Biological Sciences and Molecular Biosensor and Imaging Center, Carnegie Mellon University (USA)

  3. 3

    Molecular Biosensor and Imaging Centre, Carnegie Mellon University (USA)

  4. 4

    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA (USA)

*Department of Chemistry, Department of Biological Sciences and Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213 (USA)

  1. †

    This project was supported by grants from the National Center for Research Resources (5U54RR022241-08) and the National Institute of General Medical Sciences (8 U54 GM103529-08) from the National Institutes of Health. We would like to thank Jonathan W. Jarvik for the gift of stable ADRB2 cells, Yehuda Creeger for help with flow cytometry, and Haibing Teng for help with confocal microscopy. We would like to thank Lauren Ernst and Chris Szent-Gyorgyi for helpful discussions, and Kristen McConnell for assistance preparing the illustration. Microscopes used for the project were acquired under NIH grants 1S10RR024716 and 1S10RR026766.

Publication History

  1. Issue published online: 8 MAY 2012
  2. Article first published online: 29 MAR 2012
  3. Manuscript Revised: 19 JAN 2012
  4. Manuscript Received: 17 NOV 2011

Funded by

  • National Center for Research Resources. Grant Number: 5U54RR022241-08
  • National Institutes of Health

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Keywords:

  • analytical methods;
  • fluorescence;
  • FRET;
  • imaging agents;
  • sensors
Thumbnail image of graphical abstract

Traffic cam: A tandem dye prepared from a FRET acceptor and a fluorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization serves to follow protein trafficking during endocytosis. This sensor was used to analyze agonist-dependent internalization of β2-adrenergic receptors (see scheme). It was also used as a surrogate antigen to reveal direct surface-to-endosome antigen transfer between dendritic cells (not shown).

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