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Click-Mediated Labeling of Bacterial Membranes through Metabolic Modification of the Lipopolysaccharide Inner Core

Authors

  • Dr. Audrey Dumont,

    1. Aix Marseille Université, Laboratoire de Chimie, Bactérienne (UMR 7283), Institut de Microbiologie de la Méditerranée (IMM), CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille (France)
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  • Dr. Annie Malleron,

    1. CNRS and Université Paris-Sud, Glycochimie Moléculaire et Macromoléculaire, ICMMO, UMR 8182, 91405 Orsay (France)
    2. Laboratory of Excellence in Research on Medication and Innovative Therapeutics (LERMIT) (France)
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  • Monzer Awwad,

    1. CNRS and Université Paris-Sud, Glycochimie Moléculaire et Macromoléculaire, ICMMO, UMR 8182, 91405 Orsay (France)
    2. Laboratory of Excellence in Research on Medication and Innovative Therapeutics (LERMIT) (France)
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  • Dr. Sam Dukan,

    Corresponding author
    1. Aix Marseille Université, Laboratoire de Chimie, Bactérienne (UMR 7283), Institut de Microbiologie de la Méditerranée (IMM), CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille (France)
    • Aix Marseille Université, Laboratoire de Chimie, Bactérienne (UMR 7283), Institut de Microbiologie de la Méditerranée (IMM), CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille (France)
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  • Dr. Boris Vauzeilles

    Corresponding author
    1. CNRS and Université Paris-Sud, Glycochimie Moléculaire et Macromoléculaire, ICMMO, UMR 8182, 91405 Orsay (France)
    2. Laboratory of Excellence in Research on Medication and Innovative Therapeutics (LERMIT) (France)
    • CNRS and Université Paris-Sud, Glycochimie Moléculaire et Macromoléculaire, ICMMO, UMR 8182, 91405 Orsay (France)
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Abstract

original image

Clicking bugs: Metabolically active Gram-negative bacteria can specifically incorporate an azide-modified analogue of 3-deoxy-D-manno-octulosonic acid (1; see scheme) into the lipopolysaccharides on their membranes. This process adds a bioorthogonal chemical reporter onto the cell surface and allows the rapid fluorescent labeling of viable cells by click chemistry.

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