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Direct Fluorescence Monitoring of DNA Base Excision Repair

Authors


  • This work was supported by the U.S. National Institutes of Health (GM067201 to E.T.K. and CA67985 to S.S.D.). T.O. acknowledges a postdoctoral fellowship from JSPS.

Abstract

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Uracil is a common form of DNA damage resulting from hydrolysis of cytosine, and cellular uracil DNA glycosylases (UDG) have evolved to remove it specifically. The use of nonnatural pyrene deoxyriboside in short DNA oligomers to directly report on UDG enzymatic activity is described. The mechanism relies on the use of uracil as a strong quencher of pyrene, and enzyme repair activity can be directly imaged with bacterial cells in real time.

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