Zinc-Finger Proteins for Site-Specific Protein Positioning on DNA-Origami Structures


  • This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (Japan) to T.M. (No. 19021023 and 20241051).


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Point the finger: Zinc-finger proteins are convenient and site-selective adaptors for targeting specific locations within DNA-origami structures. Orthogonal targeting of the specific locations in the structures was demonstrated by using two adaptors, and the application of Escherichia coli lysate that contained the adaptor-fused proteins successfully afforded the expected protein–DNA assembly.