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Directing Stem Cell Fate by Controlling the Affinity and Density of Ligand–Receptor Interactions at the Biomaterials Interface

Authors

  • Prof. Kristopher A. Kilian,

    1. Department of Chemistry, Department of Cell and Molecular Biology, Department of Biomedical Engineering, Northwestern University (USA)
    2. Current address: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign (USA)
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  • Prof. Milan Mrksich

    Corresponding author
    1. Department of Chemistry, Department of Cell and Molecular Biology, Department of Biomedical Engineering, Northwestern University (USA)
    • Department of Chemistry, Department of Cell and Molecular Biology, Department of Biomedical Engineering, Northwestern University (USA)
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  • This work was supported by the National Cancer Institute of the National Institutes of Health. K.A.K. was supported by a Ruth L. Kirschstein National Research Service Award Number F32M087048 from the National Institute of General Medical Sciences of the National Institutes of Health.

Abstract

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Sticky situation: The differentiation of mesenchymal stem cells can be influenced by the affinity and density of an immobilized ligand for the integrin receptors. Cells adherent to monolayers that present the high-affinity, cyclic-RGD peptide (left) show increased expression of osteogenic markers, while cells on monolayers presenting the lower-affinity, linear-RGD peptide (right) express early markers of myogenesis at a high density and neurogenesis at a low density of the ligand.

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