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Orthogonal Riboswitches for Tuneable Coexpression in Bacteria

Authors

  • Dr. Neil Dixon,

    1. School of Chemistry & Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
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    • These authors contributed equally to this work.

  • Dr. Christopher J. Robinson,

    1. School of Chemistry & Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
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    • These authors contributed equally to this work.

  • Dr. Torsten Geerlings,

    1. School of Chemistry & Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
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  • Dr. John N. Duncan,

    1. School of Chemistry & Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
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  • Dr. Sheona P. Drummond,

    1. School of Chemistry & Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
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  • Prof. Jason Micklefield

    Corresponding author
    1. School of Chemistry & Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
    • School of Chemistry & Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
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  • This work was supported by the BBSRC (Grants BB/D005612/1 & BB/I012648/1).

Abstract

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Orthogonal gene control: Orthogonal riboswitches can be deployed in the same bacterial cell to independently control the coexpression of multiple genes in a dose-dependent response to distinct synthetic small molecules. This technique allows convenient access to highly dynamic expression landscapes and desirable protein stoichiometries.

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