Rapid, Sensitive, and Quantitative Detection of Pathogenic DNA at the Point of Care through Microfluidic Electrochemical Quantitative Loop-Mediated Isothermal Amplification

Authors

  • Kuangwen Hsieh,

    1. Department of Mechanical Engineering, University of California, Santa Barbara (USA)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Adriana S. Patterson,

    1. Department of Chemistry and Biochemistry and Biomolecular Science and Engineering Program, University of California, Santa Barbara (USA)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Dr. B. Scott Ferguson,

    1. Department of Mechanical Engineering, University of California, Santa Barbara (USA)
    Search for more papers by this author
  • Prof. Kevin W. Plaxco,

    1. Department of Chemistry and Biochemistry and Biomolecular Science and Engineering Program, University of California, Santa Barbara (USA)
    Search for more papers by this author
  • Prof. H. Tom Soh

    Corresponding author
    1. Materials Department and Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106 (USA)
    2. Department of Mechanical Engineering, University of California, Santa Barbara (USA)
    • Materials Department and Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106 (USA)
    Search for more papers by this author

  • We are grateful for the financial support from the National Institutes of Health and the Institute of Collaborative Biotechnologies through the Army Research Office. We thank Prof. Ryan White for valuable discussions. We also thank the Plaxco Lab and the Turner Lab at UCSB for assistance in device preparation. Microfabrication was carried out in the Nanofabrication Facility at UCSB.

Abstract

original image

Single-step DNA detection: A microfluidic electrochemical loop mediated isothermal amplification platform is reported for rapid, sensitive, and quantitative detection of pathogen genomic DNA at the point of care (see picture). DNA amplification was electrochemically monitored in real time within a monolithic microfluidic device, thus enabling the detection of as few as 16 copies of Salmonella genomic DNA through a single-step process in less than an hour.

Ancillary