Communication

AgI Ion Mediated Formation of a C–A Mispair by DNA Polymerases†

  1. Tatsuya Funai1,
  2. Yuki Miyazaki1,
  3. Megumi Aotani1,
  4. Eriko Yamaguchi1,
  5. Dr. Osamu Nakagawa1,
  6. Dr. Shun-ichi Wada1,
  7. Prof. Hidetaka Torigoe2,
  8. Prof. Akira Ono3,
  9. Prof. Hidehito Urata1,*

Article first published online: 29 MAY 2012

DOI: 10.1002/anie.201109191

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 51, Issue 26, pages 6464–6466, June 25, 2012

Additional Information

How to Cite

Funai, T., Miyazaki, Y., Aotani, M., Yamaguchi, E., Nakagawa, O., Wada, S.-i., Torigoe, H., Ono, A. and Urata, H. (2012), AgI Ion Mediated Formation of a C–A Mispair by DNA Polymerases . Angew. Chem. Int. Ed., 51: 6464–6466. doi: 10.1002/anie.201109191

Author Information

  1. 1

    Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094 (Japan)

  2. 2

    Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601 (Japan)

  3. 3

    Department of Material & Life Chemistry, Faculty of Engineering, Kanagawa University, 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama 221-8686 (Japan)

*Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094 (Japan)

  1. †

    This work was supported in part through a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Technology (Japan).

Publication History

  1. Issue published online: 21 JUN 2012
  2. Article first published online: 29 MAY 2012
  3. Manuscript Revised: 16 APR 2012
  4. Manuscript Received: 28 DEC 2011

Funded by

  • Ministry of Education, Science, Sports and Technology (Japan)

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Keywords:

  • DNA polymerases;
  • DNA recognition;
  • metal ion-mediated base pairs;
  • primer extension;
  • silver ions
Thumbnail image of graphical abstract

Silver turns up the A–C: In the presence of AgI ions, a DNA polymerase incorporated deoxyadenosine (from dATP) at the site opposite cytosine in the template strand to afford the full-length product (see scheme), meaning that DNA polymerases prefer a C-AgI-A base pair to the more thermodynamically stable C-AgI-C base pair.

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