We thank Dr. Guido Capitani and Dr. Vincent Olieric from PSI/Swiss Light Source for recording the diffraction data and Dr. Luc Bousset for helpful discussions. This work was supported by the Agence Nationale de la Recherche (ANR-PCV08 321323), the ETH Zurich, the Swiss National Science Foundation (grant 200020_124611), and the Centre National de la Recherche Scientifique. We acknowledge support from the Partenariat Hubert Curien Germaine de Staël and the European Commission under the Seventh Framework Program (FP7; Bio-NMR 261863).
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Communication
A Sedimented Sample of a 59 kDa Dodecameric Helicase Yields High-Resolution Solid-State NMR Spectra†
Article first published online: 27 JUN 2012
DOI: 10.1002/anie.201200779
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Gardiennet, C., Schütz, A. K., Hunkeler, A., Kunert, B., Terradot, L., Böckmann, A. and Meier, B. H. (2012), A Sedimented Sample of a 59 kDa Dodecameric Helicase Yields High-Resolution Solid-State NMR Spectra . Angew. Chem. Int. Ed., 51: 7855–7858. doi: 10.1002/anie.201200779
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Publication History
- Issue published online: 25 JUL 2012
- Article first published online: 27 JUN 2012
- Manuscript Revised: 10 APR 2012
- Manuscript Received: 28 JAN 2012
Funded by
- Agence Nationale de la Recherche. Grant Number: ANR-PCV08 321323
- ETH Zurich
- Swiss National Science Foundation. Grant Number: 200020_124611
- Centre National de la Recherche Scientifique
- Partenariat Hubert Curien Germaine de Staël
- European Commission. Grant Number: Bio-NMR 261863
Keywords:
- centrifugation;
- DNaB;
- proteins;
- sample preparation;
- solid-state NMR spectroscopy
Crystal clear: Preparing solid-state NMR samples that yield high-resolution spectra displaying high sensitivity is time-consuming and complicated. A sample of the 59 kDa protein DnaB, prepared simply by preparative centrifugation, provides spectra that are as good as the ones from carefully grown microcrystals.