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A Sedimented Sample of a 59 kDa Dodecameric Helicase Yields High-Resolution Solid-State NMR Spectra

Authors

  • Dr. Carole Gardiennet,

    1. Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/Université de Lyon1, 7 passage du Vercors, 69367 Lyon (France)
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  • Anne K. Schütz,

    1. Physical Chemistry, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich (Switzerland)
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  • Andreas Hunkeler,

    1. Physical Chemistry, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich (Switzerland)
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  • Dr. Britta Kunert,

    1. Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/Université de Lyon1, 7 passage du Vercors, 69367 Lyon (France)
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  • Dr. Laurent Terradot,

    1. Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/Université de Lyon1, 7 passage du Vercors, 69367 Lyon (France)
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  • Dr. Anja Böckmann,

    Corresponding author
    1. Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/Université de Lyon1, 7 passage du Vercors, 69367 Lyon (France)
    • Institut de Biologie et Chimie des Protéines, UMR 5086 CNRS/Université de Lyon1, 7 passage du Vercors, 69367 Lyon (France)
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  • Prof. Dr. Beat H. Meier

    Corresponding author
    1. Physical Chemistry, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich (Switzerland)
    • Physical Chemistry, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich (Switzerland)
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  • We thank Dr. Guido Capitani and Dr. Vincent Olieric from PSI/Swiss Light Source for recording the diffraction data and Dr. Luc Bousset for helpful discussions. This work was supported by the Agence Nationale de la Recherche (ANR-PCV08 321323), the ETH Zurich, the Swiss National Science Foundation (grant 200020_124611), and the Centre National de la Recherche Scientifique. We acknowledge support from the Partenariat Hubert Curien Germaine de Staël and the European Commission under the Seventh Framework Program (FP7; Bio-NMR 261863).

Abstract

original image

Crystal clear: Preparing solid-state NMR samples that yield high-resolution spectra displaying high sensitivity is time-consuming and complicated. A sample of the 59 kDa protein DnaB, prepared simply by preparative centrifugation, provides spectra that are as good as the ones from carefully grown microcrystals.

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