This work was financially supported by the “Nederlandse Organisatie voor Wetenschappelijk Onderzoek” (NWO) through the NWO-CW TOP program number 700.58.302. We further acknowledge support from the Stichting Internationaal Parkinson Fonds. We thank Prof. Alfred Meixner for help with designing and realizing the microscope.
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Communication
Molecular Composition of Sub-stoichiometrically Labeled α-Synuclein Oligomers Determined by Single-Molecule Photobleaching†
Article first published online: 13 JUL 2012
DOI: 10.1002/anie.201200813
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Zijlstra, N., Blum, C., Segers-Nolten, I. M. J., Claessens, M. M. A. E. and Subramaniam, V. (2012), Molecular Composition of Sub-stoichiometrically Labeled α-Synuclein Oligomers Determined by Single-Molecule Photobleaching . Angew. Chem. Int. Ed., 51: 8821–8824. doi: 10.1002/anie.201200813
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Publication History
- Issue published online: 22 AUG 2012
- Article first published online: 13 JUL 2012
- Manuscript Revised: 25 JUN 2012
- Manuscript Received: 30 JAN 2012
Funded by
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- NWO. Grant Number: 700.58.302
- Stichting Internationaal Parkinson Fonds
Keywords:
- α-synuclein;
- photobleaching;
- proteins;
- single-molecule studies
Bleaching proteins: Single-molecule photobleaching approaches and sub-stoichiometric labeling with fluorophores give insight into the number of monomers that form a specific α-synuclein oligomer. The results show that this α-synuclein oligomer is present as a single, well-defined species consisting of 31 monomers.