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Molecular Composition of Sub-stoichiometrically Labeled α-Synuclein Oligomers Determined by Single-Molecule Photobleaching

Authors

  • Niels Zijlstra,

    1. Nanobiophysics, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede (The Netherlands)
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  • Dr. Christian Blum,

    1. Nanobiophysics, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede (The Netherlands)
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  • Dr. Ine M. J. Segers-Nolten,

    1. Nanobiophysics, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede (The Netherlands)
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  • Dr. Mireille M. A. E. Claessens,

    1. Nanobiophysics, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede (The Netherlands)
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  • Prof. Dr. Vinod Subramaniam

    Corresponding author
    1. Nanobiophysics, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede (The Netherlands)
    • Nanobiophysics, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede (The Netherlands)
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  • This work was financially supported by the “Nederlandse Organisatie voor Wetenschappelijk Onderzoek” (NWO) through the NWO-CW TOP program number 700.58.302. We further acknowledge support from the Stichting Internationaal Parkinson Fonds. We thank Prof. Alfred Meixner for help with designing and realizing the microscope.

Abstract

original image

Bleaching proteins: Single-molecule photobleaching approaches and sub-stoichiometric labeling with fluorophores give insight into the number of monomers that form a specific α-synuclein oligomer. The results show that this α-synuclein oligomer is present as a single, well-defined species consisting of 31 monomers.

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