Development of Protein-Labeling Probes with a Redesigned Fluorogenic Switch Based on Intramolecular Association for No-Wash Live-Cell Imaging


  • This work was supported by MEXT of Japan (Grants 20675004, 22108519 to K.K. and 22685016, 23114710 to Y.H.), by the Funding Program for World-Leading Innovative R&D on Science and Technology from JSPS, by the Grant-in-Aid from the Ministry of Health, Labour and Welfare (MHLW) of Japan, by CREST from JST, and by Asahi Glass Foundation.


original image

Turn on the switch: Fluorogenic probes for protein labeling based on the photoactive yellow protein (PYP) tag were developed. The fluorescence of the probes is turned off by intramolecular association and switched on by the reversal of this interaction upon reaction with the PYP tag that is fused to the protein of interest (POI, see scheme). The rapid and specific labeling reaction enabled the imaging of cell-surface proteins without washing.