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Chemical Protein Synthesis by Chemoselective α-Ketoacid–Hydroxylamine (KAHA) Ligations with 5-Oxaproline

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Errata

This article is corrected by:

  1. Errata: Corrigendum: Chemical Protein Synthesis by Chemoselective α-Ketoacid–Hydroxylamine (KAHA) Ligations with 5-Oxaproline Volume 53, Issue 45, 12005, Article first published online: 22 September 2014

  • This work was supported by the Swiss National Science Foundation (200021_131957) and an ETH Fellowship to V.R.P. (ETH FEL 06-10-1). We are grateful to Dr. Xiangyang Zhang and Rolf Häfliger of the LOC Mass Spectrometry Service for analyses and Prof. Andrea Vasella for helpful discussions.

Abstract

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The chemical synthesis of proteins from unprotected peptide segments uses KAHA ligation with 5-oxaproline, which is incorporated readily into peptides by solid-phase peptide synthesis. Ligation of such protein segments in aqueous buffers with an α-ketoacid peptide gives an α-homoserine residue at the ligation site. The new ligation is used for the synthesis of two proteins, prokaryotic-ubiquitin like protein (Pup) and probable cold shock protein A (cspA; see scheme).

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