This research was financially supported by the Netherlands Organisation for Scientific Research (NWO) and the Netherlands Genomics Initiative (NGI).
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Communication
Triple Bioorthogonal Ligation Strategy for Simultaneous Labeling of Multiple Enzymatic Activities†
Article first published online: 21 MAR 2012
DOI: 10.1002/anie.201200923
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Willems, L. I., Li, N., Florea, B. I., Ruben, M., van der Marel, G. A. and Overkleeft, H. S. (2012), Triple Bioorthogonal Ligation Strategy for Simultaneous Labeling of Multiple Enzymatic Activities . Angew. Chem. Int. Ed., 51: 4431–4434. doi: 10.1002/anie.201200923
- †
Publication History
- Issue published online: 24 APR 2012
- Article first published online: 21 MAR 2012
- Manuscript Received: 2 FEB 2012
Funded by
- Netherlands Organisation for Scientific Research (NWO)
- Netherlands Genomics Initiative (NGI)
Keywords:
- activity-based profiling;
- bioorthogonal reactions;
- click chemistry;
- cycloadditions;
- tetrazines
Three at the same time: A ligation strategy combining tetrazine–norbornene cycloaddition, Staudinger–Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation reactions enables selective monitoring of multiple targets at the same time in complex biological samples.