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Keywords:

  • activity-based profiling;
  • bioorthogonal reactions;
  • click chemistry;
  • cycloadditions;
  • tetrazines
Thumbnail image of graphical abstract

Three at the same time: A ligation strategy combining tetrazine–norbornene cycloaddition, Staudinger–Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation reactions enables selective monitoring of multiple targets at the same time in complex biological samples.