Communication

Significantly Improved Analytical Sensitivity of Lateral Flow Immunoassays by Using Thermal Contrast†

  1. Zhenpeng Qin1,
  2. Prof. Warren C. W. Chan5,
  3. Prof. David R. Boulware4,
  4. Prof. Taner Akkin2,
  5. Elissa K. Butler4,
  6. Prof. John C. Bischof1,2,3,*

Article first published online: 23 MAR 2012

DOI: 10.1002/anie.201200997

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 51, Issue 18, pages 4358–4361, April 27, 2012

Additional Information

How to Cite

Qin, Z., Chan, W. C. W., Boulware, D. R., Akkin, T., Butler, E. K. and Bischof, J. C. (2012), Significantly Improved Analytical Sensitivity of Lateral Flow Immunoassays by Using Thermal Contrast . Angew. Chem. Int. Ed., 51: 4358–4361. doi: 10.1002/anie.201200997

Author Information

  1. 1

    Department of Mechanical Engineering, University of Minnesota, 111 Church St. SE, Minneapolis, MN 55455 (USA) http://me.umn.edu/people/bischof.shtml

  2. 2

    Department of Biomedical Engineering, University of Minnesota (USA)

  3. 3

    Department of Urology, University of Minnesota (USA)

  4. 4

    Department of Medicine, Center for Infectious Disease and Microbiology Translational Research, University of Minnesota (USA)

  5. 5

    Institute of Biomaterials & Biomedical Engineering &Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Toronto, ON (Canada)

*Department of Mechanical Engineering, University of Minnesota, 111 Church St. SE, Minneapolis, MN 55455 (USA) http://me.umn.edu/people/bischof.shtml

  1. †

    This work is supported partially by McKnight Professorship and Minnesota Futures Grant from the University of Minnesota, and NSF/CBET Grant no. 1066343(JCB). D.R.B. is supported by the National Institute of Health, NIAID K23AI073192, and E.B. is supported by NIH NIAID U01AI089244. The authors thank Greg Huan and Vincent Blonigen for the help with the thermal contrast experiment, Neha Shah for the training of GNP synthesis, Robert Hafner and Pingyan Lei for the help with TEM imaging.

Publication History

  1. Issue published online: 24 APR 2012
  2. Article first published online: 23 MAR 2012
  3. Manuscript Received: 7 FEB 2012

Funded by

  • University of Minnesota
  • NSF/CBET. Grant Number: 1066343(JCB)
  • National Institute of Health. Grant Numbers: NIAID K23AI073192, NIAID U01AI089244

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Keywords:

  • biosensors;
  • diagnostics;
  • immunoassays;
  • nanoparticles;
  • thermal contrast
Thumbnail image of graphical abstract

Heat beyond visual: The thermal contrast from the heating of gold nanoparticles upon laser stimulation can improve the analytical sensitivity of lateral flow assays (LFAs; see picture). A 32-fold improvement in sensitivity of an approved LFA for cryptococcal antigen (purple diamond) was shown, with the potential for further improvement by optimizing the backing material and the properties of the antibody-coated nanoparticles (red circle with blue Y).

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