These authors contributed equally to this work.
Hyperpolarized Binding Pocket Nuclear Overhauser Effect for Determination of Competitive Ligand Binding†
Article first published online: 12 APR 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 21, pages 5179–5182, May 21, 2012
How to Cite
Lee, Y., Zeng, H., Mazur, A., Wegstroth, M., Carlomagno, T., Reese, M., Lee, D., Becker, S., Griesinger, C. and Hilty, C. (2012), Hyperpolarized Binding Pocket Nuclear Overhauser Effect for Determination of Competitive Ligand Binding . Angew. Chem. Int. Ed., 51: 5179–5182. doi: 10.1002/anie.201201003
C.H. thanks the Camille and Henry Dreyfus Foundation for a New Faculty Award. Support from the National Science Foundation (grant number CHE-0846402), and from the Texas A&M University startup funds is gratefully acknowledged. C.G. thanks the Max Planck Society for support. This project was also performed under the auspices of the BMBF project (“Neue Verfahren der Bio-NMR zur Optimierung und Beschleunigung strukturbasierter Wirkstoffentwicklung”) and the EU funded FP7-Bio-NMR project (project number: 261863).
- Issue published online: 15 MAY 2012
- Article first published online: 12 APR 2012
- Manuscript Received: 7 FEB 2012
- Camille and Henry Dreyfus Foundation
- National Science Foundation. Grant Number: CHE-0846402
- Texas A&M University
- Max Planck Society
- EU. Grant Number: 261863
- NMR spectroscopy;
Protein-mediated polarization transfer: Ligands L1 and L2 that competitively bind to a protein are subject to indirect spin-polarization transfer through the binding site of the protein. If protons HL1 of one ligand are hyperpolarized by dynamic nuclear polarization (DNP, see picture), signal intensities in the NMR spectrum of the second ligand become enhanced. The relative build-up of signal of the second ligand yields information on its binding epitope.