• Open Access

Raman Spectroscopic Detection of Anthrax Endospores in Powder Samples

Authors

  • S. Stöckel,

    1. Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, 07743 Jena (Germany)
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  • S. Meisel,

    1. Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, 07743 Jena (Germany)
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  • Dr. M. Elschner,

    1. Friedrich Loeffler Institut, Bundesforschungsinstitut für Tiergesundheit, Institut für bakterielle Infektionen und Zoonosen, Naumburger Strasse 96a, 07743 Jena (Germany)
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  • Dr. P. Rösch,

    1. Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, 07743 Jena (Germany)
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  • Prof. Dr. J. Popp

    Corresponding author
    1. Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, 07743 Jena (Germany)
    2. Institut für Photonische Technologien, Albert-Einstein-Strasse 9, 07745 Jena (Germany)
    • Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, 07743 Jena (Germany)
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  • Funding of the research projects “Pathosafe” (FKZ 13N9547 and FKZ 13N9549) and “RamaDek” (FKZ 13N11168) by the Federal Ministry of Education and Research (Germany) (BMBF) and “MikroPlex” (PE113-1) by the Thüringische Exzellenzinitiative (TMBWK) as well as funding by the EU, EAHC Agreement (No. 2007 204) is gratefully acknowledged.

Abstract

original image

Better safe than sorry: A combined analytical procedure consisting of Raman spectroscopy and chemometrics can be used to test for anthrax endospores in environmental samples. Time-consuming enrichment steps are not required and low levels of anthrax endospores in household substances like baking powder can be detected within 3 h and discriminated against other nonpathogenic Bacillus species with typing accuracies of up to 99 %.

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