Protein Expression, Aggregation, and Triggered Release from Polymersomes as Artificial Cell-like Structures

Authors

  • Chiara Martino,

    1. The Division of Biomedical Engineering, School of Engineering, The University of Glasgow, Glasgow, G12 8LT (UK)
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    • These authors contributed equally to this work.

  • Dr. Shin-Hyun Kim,

    1. School of Engineering and Applied Sciences, Department of Physics, Harvard University, Cambridge, MA 02138 (USA)
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    • These authors contributed equally to this work.

  • Dr. Louise Horsfall,

    1. The Institute of Molecular Cell and System Biology, College of Medical and Veterinary and Life Science, The University of Glasgow, Glasgow, G12 8QQ (UK)
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  • Dr. Alireza Abbaspourrad,

    1. School of Engineering and Applied Sciences, Department of Physics, Harvard University, Cambridge, MA 02138 (USA)
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  • Dr. Susan J. Rosser,

    1. The Institute of Molecular Cell and System Biology, College of Medical and Veterinary and Life Science, The University of Glasgow, Glasgow, G12 8QQ (UK)
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  • Prof. Jonathan Cooper,

    Corresponding author
    1. The Division of Biomedical Engineering, School of Engineering, The University of Glasgow, Glasgow, G12 8LT (UK)
    • The Division of Biomedical Engineering, School of Engineering, The University of Glasgow, Glasgow, G12 8LT (UK)
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  • Prof. David A. Weitz

    Corresponding author
    1. School of Engineering and Applied Sciences, Department of Physics, Harvard University, Cambridge, MA 02138 (USA)
    • School of Engineering and Applied Sciences, Department of Physics, Harvard University, Cambridge, MA 02138 (USA)
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  • We are grateful to BBSRC for support of the work, Tianjin University for the preparation of the MreB-RFP sequence, and MacRobertson Scholarship, University of Glasgow (for student mobility). This work was supported by Amore-Pacific, the NSF (DMR-1006546), and the Harvard MRSEC (DMR-0820484).

Abstract

original image

Bringing droplets to life: A cytoskeletal protein (red dots, see scheme) is expressed in artificial cells composed of biocompatible polymersomes, which encapsulate expression machinery and amino acid building blocks. Release of the expressed proteins can be triggered by a negative osmotic shock.

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