These authors contributed equally to this work and are listed in alphabetical order of their surnames.
Benzoylphosphonate-Based Photoactive Phosphopeptide Mimetics for Modulation of Protein Tyrosine Phosphatases and Highly Specific Labeling of SH2 Domains†
Article first published online: 21 AUG 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 37, pages 9441–9447, September 10, 2012
How to Cite
Horatscheck, A., Wagner, S., Ortwein, J., Kim, B. G., Lisurek, M., Beligny, S., Schütz, A. and Rademann, J. (2012), Benzoylphosphonate-Based Photoactive Phosphopeptide Mimetics for Modulation of Protein Tyrosine Phosphatases and Highly Specific Labeling of SH2 Domains . Angew. Chem. Int. Ed., 51: 9441–9447. doi: 10.1002/anie.201201475
This work was supported by the DFG (FOR 806, SFB 765) and the Investitionsbank Berlin, ProFit-Projekt 10143721. MptpA was provided by Dr. Matthew Groves, EMBL-HH, STAT5b by Dr. Anne Diehl, FMP, using a clone donated by Prof. Thorsten Berg, Leipzig. We thank Natalja Erdmann, Janett Tischer, and Tracy Dornblut for excellent technical assistance.
- Issue published online: 5 SEP 2012
- Article first published online: 21 AUG 2012
- Manuscript Revised: 31 MAY 2012
- Manuscript Received: 22 FEB 2012
- DFG. Grant Numbers: FOR 806, SFB 765
- Investitionsbank Berlin. Grant Number: 10143721
- phosphotyrosine isosters;
- photoaffinity labeling;
- protein tyrosine phosphatases
A light switch for phosphotyrosine- recognizing proteins: Irradiation of the bioisosteric benzoylphosphonate suffices to “turn off” the activity of target proteins and to label them covalently (see scheme). Photoactive bioisosters may find applications in functional cell biology, bioanalytics, and proteome research.