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Benzoylphosphonate-Based Photoactive Phosphopeptide Mimetics for Modulation of Protein Tyrosine Phosphatases and Highly Specific Labeling of SH2 Domains


  • This work was supported by the DFG (FOR 806, SFB 765) and the Investitionsbank Berlin, ProFit-Projekt 10143721. MptpA was provided by Dr. Matthew Groves, EMBL-HH, STAT5b by Dr. Anne Diehl, FMP, using a clone donated by Prof. Thorsten Berg, Leipzig. We thank Natalja Erdmann, Janett Tischer, and Tracy Dornblut for excellent technical assistance.


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A light switch for phosphotyrosine- recognizing proteins: Irradiation of the bioisosteric benzoylphosphonate suffices to “turn off” the activity of target proteins and to label them covalently (see scheme). Photoactive bioisosters may find applications in functional cell biology, bioanalytics, and proteome research.