Drawing Sticky Adeno-Associated Viruses on Surfaces for Spatially Patterned Gene Expression


  • This work was supported by National Research Foundation (NRF) grant funded by the Korea government (MEST) through the Active Polymer Center for Pattern Integration (No. R11-2007-050-00000-0 to J.H.J.), the Human Resources Development of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government Ministry of Knowledge Economy (No. 20104010100500 to J.H.J.), Pioneer Research Program, (2011-0001696 to H.L.), and Korea Biotech R&D Program (2011K000809 to H.L.).


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Sticky stuff: A versatile strategy to spatially control gene expressions of mammalian cells is developed. A catecholamine polymer (PEI-C) is used to functionalize surfaces of adeno-associated viruses (AAV). Because of the underwater adhesive property of catechol, AAV/PEI-C hybrid vectors become highly “sticky”, resulting in spatially patterned viral attachment onto substrates by the simple “gene-vector drawing” technique (see picture).