Communication

Polyspermine Imidazole-4,5-imine, a Chemically Dynamic and Biologically Responsive Carrier System for Intracellular Delivery of siRNA†

  1. Shiyue Duan,
  2. Weien Yuan,
  3. Fei Wu,
  4. Tuo Jin*

Article first published online: 16 JUL 2012

DOI: 10.1002/anie.201201793

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 51, Issue 32, pages 7938–7941, August 6, 2012

Additional Information

How to Cite

Duan, S., Yuan, W., Wu, F. and Jin, T. (2012), Polyspermine Imidazole-4,5-imine, a Chemically Dynamic and Biologically Responsive Carrier System for Intracellular Delivery of siRNA. Angew. Chem. Int. Ed., 51: 7938–7941. doi: 10.1002/anie.201201793

Author Information

  1. Laboratory of Drug Delivery and Biomaterials, School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai, 200240 (P.R. China)

*Laboratory of Drug Delivery and Biomaterials, School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai, 200240 (P.R. China)

  1. †

    We thank the Ministry of Science and Technology (2009ZX09310-007) of the People’s Republic of China and the Technology Committee of Science and Technology Special Nanotechnology Grant (No. 1052nm03900) for financial support. We also acknowledge Prof. Y. H. Xu (School of Pharmacy, Shanghai Jiao Tong University) for providing SMMC-7721 cells stably expressing the pGL3 luciferase gene.

Publication History

  1. Issue published online: 1 AUG 2012
  2. Article first published online: 16 JUL 2012
  3. Manuscript Revised: 18 MAY 2012
  4. Manuscript Received: 6 MAR 2012

Funded by

  • Ministry of Science and Technology. Grant Number: 2009ZX09310-007
  • Technology Committee of Science and Technology Special Nanotechnology Grant. Grant Number: 1052nm03900

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Keywords:

  • biodegradable polymers;
  • cytotoxicity;
  • drug delivery;
  • siRNA;
  • spermine
Thumbnail image of graphical abstract

Silence please! Polyspermine imidazole-4,5-imine (blue lines) was formed by condensing spermine with bisformaldehyde imidazole through a pH-responsive linkage. This polymer was used to condense siRNAs into nanoparticles (see scheme) and studied for delivery into cells and release from endosomes. Cellular and in vivo assays indicated that this siRNA carrier was efficient in silencing target genes with negligible cytotoxicity.

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