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Keywords:

  • cell selection;
  • chitin-binding domain;
  • gene expression;
  • protein engineering;
  • transfection
Thumbnail image of graphical abstract

Vectors have been constructed that express the chitin-binding domain (ChBD) on eukaryotic cell surfaces. The ChBD is linked to enhanced green fluorescent protein (EGFP) through a protein that spans the plasma membrane (see picture). This binding functionality does not have a counterpart in eukaryotes, thereby endowing the modified cell surface with a property that is orthogonal to animal cells.