This work was supported by the U.S. National Institutes of Health (GM067201). We thank Dr. Lindsey E. McQuade and Prof. James K. Chen for assistance and advice with zebrafish experiments, and Prof. Ingmar H. Riedel-Kruse for helpful advice on Paramecium culture.
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Communication
DNA Polyfluorophores for Real-Time Multicolor Tracking of Dynamic Biological Systems†
Article first published online: 8 JUN 2012
DOI: 10.1002/anie.201201928
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Wang, S., Guo, J., Ono, T. and Kool, E. T. (2012), DNA Polyfluorophores for Real-Time Multicolor Tracking of Dynamic Biological Systems . Angew. Chem. Int. Ed., 51: 7176–7180. doi: 10.1002/anie.201201928
- †
Publication History
- Issue published online: 11 JUL 2012
- Article first published online: 8 JUN 2012
- Manuscript Revised: 16 APR 2012
- Manuscript Received: 11 MAR 2012
Funded by
- U.S. National Institutes of Health. Grant Number: GM067201
Keywords:
- fluorescence;
- imaging agents;
- multicolor;
- oligonucleotides;
- real-time tracking
Dye-ing to live: Spectral limitations of common organic dyes make it difficult or impossible to visualize and follow multiple biological components in rapidly moving systems. The development of a multispectral set of improved DNA-scaffolded fluorophores is described. Their use in multicolor cellular imaging (see scheme) and in tracking of biological motions on the subsecond timescale is demonstrated.