These authors contributed equally to this work.
A Native-Like Conformation for the C-Terminal Domain of the Prion Ure2p within its Fibrillar Form†
Article first published online: 3 JUL 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 32, pages 7963–7966, August 6, 2012
How to Cite
Habenstein, B., Bousset, L., Sourigues, Y., Kabani, M., Loquet, A., Meier, B. H., Melki, R. and Böckmann, A. (2012), A Native-Like Conformation for the C-Terminal Domain of the Prion Ure2p within its Fibrillar Form. Angew. Chem. Int. Ed., 51: 7963–7966. doi: 10.1002/anie.201202093
This work was supported by the Agence Nationale de la Recherche (ANR-07-PCVI-0013-03, ANR-06-BLAN-0266, ANR-PCV08 321323, and ANR08-PCVI-0022-02), the ETH Zurich, the Swiss National Science Foundation (Grant 200020_124611), and the Centre National de la Recherche Scientifique. We acknowledge support from the Partenariat Hubert Curien Germaine de Staël. We also acknowledge support from the European Commission under the Seventh Framework Programme (FP7), contract Bio-NMR 261863. We also thank Dr. Christian Wasmer for help in setting up the experiments.
- Issue published online: 1 AUG 2012
- Article first published online: 3 JUL 2012
- Manuscript Revised: 30 APR 2012
- Manuscript Received: 16 MAR 2012
- Agence Nationale de la Recherche. Grant Numbers: ANR-07-PCVI-0013-03, ANR-06-BLAN-0266, ANR-PCV08 321323, ANR08-PCVI-0022-02
- ETH Zurich
- Swiss National Science Foundation. Grant Number: 200020_124611
- Centre National de la Recherche Scientifique
- European Commission
- NMR spectroscopy;
- protein structures;
- solid-state NMR spectroscopy;
Taking a definite stance: Protein fibrils are often associated with disorder and polymorphism, but the prion fibrils of Ure2p are shown (through solid-state NMR spectroscopy) to be highly ordered, and the conformations of the globular domain to be more restricted within the fibrils (black; see scheme) than in Ure2p single crystals (red). This finding implies that steric impairment is at the origin of the [URE3] phenotype in yeast.