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Keywords:

  • enzymes;
  • infrared spectroscopy;
  • protein dynamics;
  • reaction mechanism;
  • ultrafast spectroscopy
Thumbnail image of graphical abstract

The role of protein dynamics in promoting catalysis is hotly debated. Infrared data from both ultrafast flash photolysis and stopped-flow studies show that not only does there appear to be vibrational coupling between the cofactor and protein in B12-dependent ethanolamine ammonia lyase, but also that there are significant protein motions coupled to the reaction that follows substrate binding (see picture).