Communication

Mechanism and Stem-Cell Activity of 5-Carboxycytosine Decarboxylation Determined by Isotope Tracing†

  1. Stefan Schiesser1,‡,
  2. Benjamin Hackner1,‡,
  3. Toni Pfaffeneder1,
  4. Markus Müller1,
  5. Dr. Christian Hagemeier2,
  6. Dr. Matthias Truss2,*,
  7. Prof. Dr. Thomas Carell1,*

Article first published online: 29 MAY 2012

DOI: 10.1002/anie.201202583

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 51, Issue 26, pages 6516–6520, June 25, 2012

Additional Information

How to Cite

Schiesser, S., Hackner, B., Pfaffeneder, T., Müller, M., Hagemeier, C., Truss, M. and Carell, T. (2012), Mechanism and Stem-Cell Activity of 5-Carboxycytosine Decarboxylation Determined by Isotope Tracing . Angew. Chem. Int. Ed., 51: 6516–6520. doi: 10.1002/anie.201202583

Author Information

  1. 1

    Center for Integrated Protein Science at the Department of Chemistry, Ludwig-Maximilians Universität München, Butenandtstrasse 5–13, 81377 München (Germany)

  2. 2

    Charité Universitätsklinikum, Otto-Heubner-Centrum für Kinder und Jugendmedizin, Klinik für Allgemeine Pädiatrie, Labor für Pädiatrische Molekularbiologie, Ziegelstrasse 5–9, 10098 Berlin (Germany)

  1. ‡

    These authors contributed equally to this work.

*Charité Universitätsklinikum, Otto-Heubner-Centrum für Kinder und Jugendmedizin, Klinik für Allgemeine Pädiatrie, Labor für Pädiatrische Molekularbiologie, Ziegelstrasse 5–9, 10098 Berlin (Germany)

  1. †

    We thank the Excellence cluster CiPSM and the collaborative research center SFB749 as well as the DFG, grant CA275/8-4, the Volkswagen Foundation and NGFNplus (01GS0870) for financial support. T.P. and S.S. thank the Fonds der Chemischen Industrie for pre-doctoral fellowships.

  2. ‡

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 21 JUN 2012
  2. Article first published online: 29 MAY 2012
  3. Manuscript Received: 3 APR 2012

Funded by

  • DFG. Grant Number: CA275/8-4
  • Volkswagen Foundation
  • NGFNplus. Grant Number: 01GS0870
  • Fonds der Chemischen Industrie

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Keywords:

  • 5-carboxycytosine;
  • decarboxylation;
  • epigenetics;
  • isotopic labeling;
  • stem cells
Thumbnail image of graphical abstract

Eraserhead: Stem cells seem to erase epigenetic information by decarboxylation of the newly discovered epigenetic base 5-carboxycytosine (caC; see picture). This reaction is likely to involve a nucleophilic attack of the C5[BOND]C6 double bond.

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